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منتج #:
71679097
DMEM/F-12 Cell Culture Medium
86% من المشترين سيوصون بهذا المنتج لصديق
منتج #:
71679097
IQD 50840
تفاصيل السعر
باستثناء رسوم الشحن والجمارك ( سيتم احتساب رسوم الشحن والجمارك عند إتمام الشراء )
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مستورد من متجر USA
كمية:
اطلب الآن واحصل عليه حول الجمعة, سبتمبر 11
معاملة آمنة
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أفضل شركائنا اللوجستيين
DMEM/F-12 is widely used for supporting the growth of many different mammalian cells
شحن
سريع
استرجاع
مجاني*
تغليف آمن
منتجات أصلية %100
الامتثال لمعيار PCI DSS
حاصل على شهادة ISO 27001
Note: Step Down Voltage Transformer required for using electronics products of أمريكا store (110-120). Recommended power converters اشتري الآن.
مايفيد
Versatile Formula
Combines DMEM and F-12 nutrients, supporting a wide range of cell types for various research applications, from adherent cells to suspension cultures.
Optimized Growth
Enhanced recipe fosters superior cell proliferation and maintenance, addressing common issues faced by researchers with slower growth rates in standard media.
Comprehensive Nutrient Mix
Includes essential vitamins, amino acids, and salts, ensuring cells receive all necessary components for optimal health and functionality, setting it apart from many competitor products.
تفاصيل المنتج
- DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12, containing amino acids, vitamins, inorganic salts, and other components necessary for multi-type cell culture.
- Widely used for supporting the growth of various mammalian cells, including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
- Does not contain proteins, lipids, or growth factors, hence supplementation with 10% Fetal Bovine Serum (FBS) is common.
- Requires a 5–10% CO2 environment to maintain physiological pH due to its sodium bicarbonate buffer system.
- Manufactured at a cGMP-compliant facility, using strict quality control testing for raw material components, and undergoes rigorous quality checks including sterility, pH, osmolality, endotoxin, and cell culture tests.
- Hygia cell culture media are produced using water for injection, filtered through 0.1 μm sterile filtration and aseptically filled in a Hundred-stage FFU with integrity tests being conducted before and after filtration.
| Date First Available | January 31, 2023 |
| Manufacturer | Hygia Reagents |
| Brand Name | Hygia Reagents |
| Unit Count | 1 Count |
| UPC | 755457565786 |
| Included Components | 1 bottle |
| Part Number | HCM-DMEM |
| Item Weight | 2 lbs (910 grams) |
من يجب أن يشتري؟
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Cell Biologists
Ideal for researchers studying various cell types in vitro, supporting diverse growth and differentiation protocols.
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Tissue Engineering
Suitable for applications in tissue engineering where cell growth and maintenance are critical for developing artificial tissues.
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Pharmaceutical Research
Beneficial for drug discovery and testing, providing a stable environment for human and animal cells during experimentation.
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Plant Cell Cultures
Not suitable for plant cell cultures which require specific nutrients and growth factors not found in DMEM/F-12.
وصف المنتج
DMEM/F-12 Cell Culture Medium
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أسئلة العملاء & الإجابات
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سؤال:
What is DMEMF-12 Cell Culture Medium used for?
إجابه: DMEMF-12 Cell Culture Medium is primarily used for the growth and maintenance of mammalian cells in laboratory settings. It is specifically formulated to support the nutritional needs of fast-growing cells, providing essential amino acids, vitamins, and minerals. Researchers commonly utilize this medium in experiments involving cell proliferation, genetic engineering, and drug development, ensuring optimal conditions for cell viability and functionality. -
سؤال:
How do you prepare DMEMF-12 Cell Culture Medium?
إجابه: Preparing DMEMF-12 Cell Culture Medium involves reconstituting the powdered form with sterile distilled water or a suitable buffer. It is important to adjust the pH to around 7.2-7.4, which provides the necessary environment for cell growth. Additionally, supplementing the medium with fetal bovine serum or other growth factors enhances its effectiveness. This preparation is crucial as it ensures that the cells receive the right nutrients and maintain their health during experiments. -
سؤال:
Can DMEMF-12 be used for primary cell cultures?
إجابه: Yes, DMEMF-12 Cell Culture Medium can be utilized for primary cell cultures, particularly for a variety of mammalian cells. Its formulation is designed to cater to the growth requirements of both established cell lines and primary cells. Its versatility makes it ideal for researchers conducting studies on tissue regeneration, cellular response, and metabolic processes in a closer-to-native environment. -
سؤال:
What makes DMEMF-12 different from other culture media?
إجابه: DMEMF-12 differentiates itself from other culture media by its unique blend of nutrients tailored for fast-growing cells. Its composition allows for better cell attachment, proliferation, and metabolic activity compared to standard DMEM. Researchers select DMEMF-12 for high-cell-density applications, ensuring improved experimental outcomes in studies related to cancer research, regenerative medicine, and pharmacological testing. -
سؤال:
Is DMEMF-12 suitable for serum-free culture?
إجابه: DMEMF-12 can be adapted for serum-free culture by supplementing it with specific growth factors and hormones tailored to the cell line being used. This serum-free approach minimizes variability and allows for a more defined environment. Many researchers prefer serum-free conditions for regulatory and consistency reasons, especially when conducting assays or scales relevant to biotech applications. -
سؤال:
How should DMEMF-12 Cell Culture Medium be stored?
إجابه: DMEMF-12 Cell Culture Medium should be stored in a cool, dry place away from direct sunlight. Once prepared, it is best to store the medium at 2-8 degrees Celsius for short-term use or freeze it for long-term storage. Proper storage is essential to maintain its integrity and effectiveness, ensuring that the cells receive the necessary nutrients and conditions for optimal growth. -
سؤال:
What cell lines are commonly grown in DMEMF-12?
إجابه: Commonly used cell lines such as HEK293, CHO, and various cancer cell lines thrive in DMEMF-12 Cell Culture Medium. These cell lines are often employed in genetic, pharmaceutical, and toxicological studies. Researchers choose DMEMF-12 to support robust growth and high cell yield, which is crucial for experiments requiring significant cell quantities for analysis. -
سؤال:
Can DMEMF-12 be modified or supplemented?
إجابه: DMEMF-12 can be modified or supplemented to better suit specific cell growth needs. Researchers might add components such as L-glutamine, non-essential amino acids, or specialized growth factors depending on the cell type. Customization allows for greater control over growth conditions, enabling researchers to optimize their cultures for experiments related to gene expression, cell signaling, and drug response. -
سؤال:
What are the signs of spoiled DMEMF-12 Medium?
إجابه: Signs of spoiled DMEMF-12 Medium include changes in color, presence of precipitate, or an unusual odor. Additionally, if cell cultures show poor growth or abnormal morphology after media change, it may indicate spoilage. For consistent and reproducible results, it is imperative to routinely check the medium for these indicators and replace it when necessary to maintain cell health and experimental accuracy. -
سؤال:
Where can I buy DMEMF-12 Cell Culture Medium in Iraq?
إجابه: You can purchase DMEMF-12 Cell Culture Medium through Ubuy in Iraq. Ubuy offers a wide range of laboratory supplies and ensures that you can find the specific formulations you need for your research. With various products available, Ubuy serves as a convenient platform for researchers looking to acquire high-quality cell culture media.
Hygia Reagents Analytical Reagents Editorial Review
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إيجابيات
- High nutrient content
- Supports a variety of cell types
- Reliable for long-term cultures
- Excellent pH stability over time
- Easy to prepare and use
سلبيات
- Occasional sediment formation
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“Good supply of products. Safe payment methods, and shipment worldwide! Genuine products.”
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الخروج الآمن
شحن عالمي
إسترجاع سهل
منتجات أصلية
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IQD 50840
اطلب الآن واحصل عليه حول الجمعة, سبتمبر 11
هذا المنتج غير ممنوع في بلدي. (الرجاء الضغط على الرابط أعلاه إذا لم يكن هذا المنتج ممنوعاً في بلدك ، لذلك سيقوم فريقنا بمراجعته والسماح به.)
كمية:
معاملة آمنة
نوفر لك مدفوعات مشفّرة، وحماية متكاملة للمشتري، مع الالتزام بمعايير PCI DSS وشهادة ISO 27001:2022 لضمان أعلى مستويات الأمان في كل عملية شراء.
أفضل شركائنا اللوجستيين
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المميزات والفوائد
- DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12
- It contains amino acids, vitamins, inorganic salts and other components needed for multi-type cell culture.
- DMEM/F-12 contains no proteins, lipids, or growth factors which requires additional supplementation.
- Hygia’s cell culture media products are screened through strict quality control testing.
- Manufactured at a cGMP-compliant facility using sterilized water.
- Each lot of DMEM/F-12 is subjected to multiple tests to ensure the highest quality.
ضمان Ubuy
تسوّق بثقة مع منتجات أصلية %100، ومدفوعات آمنة متوافقة مع معيار PCI DSS، وحماية بيانات معتمدة وفق ISO 27001، وشحن دولي سريع، وإرجاع مجاني*، وتغليف آمن لكل طلب.