UltraSensitive TMB HRP Aqueous Substrate 1-Component, Universal (50ml)
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UltraSensitive TMB HRP Aqueous Substrate 1-Component, Universal (50ml)
Ultrasensitive detection of horseradish peroxidase (HRP) activity with a color change from blue to yellow.
UltraSensitive TMB HRP Aqueous Substrate 1-Component, Universal (50ml)
منتج #: 200973946

UltraSensitive TMB HRP Aqueous Substrate 1-Component, Universal (50ml)

منتج #: 200973946

IQD 56071

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Ultrasensitive detection of horseradish peroxidase (HRP) activity with a color change from blue to yellow.
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مايفيد

Ready-to-Use Solution
This 1-component substrate eliminates the need for complex mixing, providing a hassle-free experience for users while ensuring consistent results in various applications, including immunoassays and enzyme-linked assays.
Wide Compatibility
Designed to work universally with multiple HRP (Horseradish Peroxidase) detection systems, this substrate enhances versatility, making it suitable for a broad range of experimental setups and techniques.
Enhanced Sensitivity
Offers superior sensitivity and signal amplification, enabling the detection of low-abundance targets effectively, which is critical for researchers aiming for reliable and precise data in their assays.

تفاصيل المنتج

Shop UltraSensitive TMB HRP Aqueous Substrate 1-Component, Universal (50ml) online at a best price in العراق.
Country of OriginThis item will be imported from US
Item Weight0.11 lbs (50 grams)
Date First AvailableNovember 19, 2021
What is in the boxUltraSensitive TMB HRP Aqueous... For more details, please check description/product details

التفاصيل الفنية للمنتج

Manufacturer‎RxBiosciences

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Suitable For
  • Research Laboratories

    Ideal for academic and corporate laboratories conducting enzyme-linked immunosorbent assays (ELISAs) with sensitive detection requirements.

  • Quality Control

    Suitable for industries requiring precise and sensitive quantitative measurements in product testing and quality assurance.

  • Clinical Diagnostics

    Beneficial for clinical settings performing diagnostic tests that rely on HRP enzyme-substrate reactions for accuracy.

Not Suitable For
  • Casual Users

    Not suitable for hobbyists or individuals without specialized knowledge in laboratory techniques for enzyme reactions.

وصف المنتج

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أسئلة العملاء & الإجابات

  • سؤال: What is UltraSensitive TMB HRP Aqueous Substrate used for?

    إجابه: UltraSensitive TMB HRP Aqueous Substrate is primarily used in enzyme-linked immunosorbent assays (ELISAs) for the detection of HRP (horseradish peroxidase) activity. It enables colorimetric detection of antigens or antibodies, making it essential for various applications in diagnostic and research laboratories. This substrate reacts with HRP to produce a blue color that then changes to yellow upon the addition of stop solution, allowing for quantification. It's especially useful for sensitive assays where accurate detection is crucial.
  • سؤال: How does UltraSensitive TMB HRP Aqueous Substrate work?

    إجابه: The substrate works by acting as a chromogenic reagent that HRP catalyzes to produce a color change. When HRP is present, it catalyzes the oxidation of the TMB substrate, leading to the formation of a colored product that can be measured spectrophotometrically. The reaction is pH-sensitive, ensuring that it performs optimally when stored and used according to manufacturer guidelines. Laboratories rely on this substrate for fine-tuned measurement of biological samples involving enzyme activity.
  • سؤال: What are the storage conditions for UltraSensitive TMB HRP Aqueous Substrate?

    إجابه: To ensure peak performance, UltraSensitive TMB HRP Aqueous Substrate should be stored in a cool, dark place, ideally at temperatures between 2-8 degrees Celsius. Avoid exposure to light to prevent degradation of the substrate. Proper storage extends the shelf life and maintains the substrate’s reactivity, essential for achieving reproducible results in your assays. Use within a reasonable timeframe after opening to ensure optimal performance in your experiments.
  • سؤال: Is UltraSensitive TMB HRP Aqueous Substrate suitable for all types of assays?

    إجابه: UltraSensitive TMB HRP HRP Aqueous Substrate is versatile, suitable for a wide range of assays, particularly those that are sensitive or require high specificity. It is ideal for both qualitative and quantitative assays where enzyme activity detection is critical. However, it is important to optimize conditions based on the specific assay requirements, including choose the right dilutions and incubation times, to achieve the best outcomes in your research.
  • سؤال: Can UltraSensitive TMB HRP Aqueous Substrate be used in conjunction with other detection methods?

    إجابه: Yes, UltraSensitive TMB HRP Aqueous Substrate can be used alongside other detection methods in multiplex assays, allowing for simultaneous measurements of multiple targets. Its compatibility with various antibodies and assay formats enhances its utility in complex research settings. However, it is essential to validate that the substrates or detection methods used alongside do not interfere with the TMB reaction to obtain accurate results.
  • سؤال: What makes UltraSensitive TMB HRP Aqueous Substrate different from other substrates?

    إجابه: UltraSensitive TMB HRP Aqueous Substrate distinguishes itself through its exceptional sensitivity and stability compared to traditional substrates. Its formulation allows for clearer colorimetric readings, crucial for detecting low levels of target proteins. This makes it a preferred choice in fields such as clinical diagnostics and research where precision and reliability are paramount. Users often cite its high signal-to-noise ratio as a significant advantage for achieving accurate measurements in their applications.
  • سؤال: What is the recommended usage protocol for this substrate?

    إجابه: For optimal results with UltraSensitive TMB HRP Aqueous Substrate, start by preparing your samples and controls as per your assay protocol. Add the substrate directly to wells after the HRP-linked antigen/antibody reaction. Allow it to incubate for the recommended duration as specified by your assay conditions, usually 10-30 minutes, then stop the reaction with a stop solution. Reading the absorbance promptly ensures accurate quantification of your sample's signal, allowing effective comparison to standard curves or controls.
  • سؤال: What precautions should I take when using UltraSensitive TMB HRP Aqueous Substrate?

    إجابه: While using UltraSensitive TMB HRP Aqueous Substrate, it's essential to avoid contamination and ensure all glassware and well plates are clean. Use gloves and avoid direct exposure to light during the assay to protect the substrate’s reactivity. Additionally, ensure that the substrate is equilibrated to room temperature before use and that you adhere to the specified timing for each step to maintain consistency across experiments. Following these precautions can significantly reduce experimental variability.
  • سؤال: What types of samples can be tested with UltraSensitive TMB HRP Aqueous Substrate?

    إجابه: UltraSensitive TMB HRP Aqueous Substrate is compatible with various biological samples, including serum, plasma, cell lysates, or any sample containing HRP-labeled antibodies. Particularly beneficial in detecting low-abundance proteins, it provides results that are reliable and reproducible. In scenarios such as disease biomarker studies or immunological assays, this substrate serves a vital role in obtaining quantitative insights from the samples processed.
  • سؤال: Where can I buy UltraSensitive TMB HRP Aqueous Substrate 1-Component, Universal 1000ml in Iraq?

    إجابه: You can purchase UltraSensitive TMB HRP Aqueous Substrate 1-Component, Universal 1000ml through Ubuy in Iraq. Ubuy is a reputable e-commerce platform that offers a wide range of laboratory supplies and reagents, including this substrate, making it convenient for researchers to order essential products for their experiments. You can find this item along with other lab essentials, ensuring you have everything you need in one place.

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IQD 56071

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المميزات والفوائد

  • Ready-to-use formulation for convenience.
  • Non-carcinogenic and free from toxic products.
  • Stable for 2-3 years at 4°C.
  • Shipped at room temperature for optimal condition.
  • Ultrasensitive for accurate color development.
  • Ideal for ELISA applications.

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